Key Product Details

Validated by

Biological Validation

Species Reactivity

Non-species specific

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 10A6

Concentration

1 mg/ml

View all Dityrosine Antibodies »

Product Specifications

Immunogen

Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).

Specificity

Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Dityrosine Antibody (10A6)

Western Blot: Dityrosine Antibody (10A6) [NBP2-59361]

Western Blot: Dityrosine Antibody (10A6) [NBP2-59361]

Western Blot: Dityrosine Antibody (10A6) [NBP2-59361] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Dityrosine protein using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6 (NBP2-59361). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody (NBP2-59361) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.
Immunocytochemistry/ Immunofluorescence: Dityrosine Antibody (10A6) [NBP2-59361]

Immunocytochemistry/ Immunofluorescence: Dityrosine Antibody (10A6) [NBP2-59361]

Immunocytochemistry/Immunofluorescence: Dityrosine Antibody (10A6) [NBP2-59361] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibodyat 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Dityrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Flow Cytometry: Dityrosine Antibody (10A6) [NBP2-59361]

Flow Cytometry: Dityrosine Antibody (10A6) [NBP2-59361]

Flow Cytometry: Dityrosine Antibody (10A6) [NBP2-59361] - Flow Cytometry analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6 (NBP2-59361). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody (NBP2-59361) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 M H2O2 for 24 hours.
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Applications for Dityrosine Antibody (10A6)

Application
Recommended Usage

ELISA

1:1000

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Dityrosine

Alternate Names

Dityrosine;Dityrosine (DT);DT;DY

Additional Dityrosine Products

Product Documents for Dityrosine Antibody (10A6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Dityrosine Antibody (10A6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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