DjC9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33997

Novus Biologicals, part of Bio-Techne
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NBP2-33997
NBP2-33997-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DjC9 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: EPRIRNIIQQAIDAGEVPSYNAFVKESKQKMNARKRRAQEEAKEAEMSRKELGLDEGVDSLKAAIQSRQKDRQKEMDNFLAQMEAKYCKS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DjC9 Antibody

Western Blot: DjC9 Antibody [NBP2-33997]

Western Blot: DjC9 Antibody [NBP2-33997]

Western Blot: DjC9 Antibody [NBP2-33997] - Analysis using Anti-DNAJC9 antibody NBP2-33997 (A) shows similar pattern to independent antibody NBP1-87903 (B).
Immunocytochemistry/ Immunofluorescence: DjC9 Antibody [NBP2-33997]

Immunocytochemistry/ Immunofluorescence: DjC9 Antibody [NBP2-33997]

Immunocytochemistry/Immunofluorescence: DjC9 Antibody [NBP2-33997] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm, plasma membrane & cytosol.
Immunohistochemistry-Paraffin: DjC9 Antibody [NBP2-33997]

Immunohistochemistry-Paraffin: DjC9 Antibody [NBP2-33997]

Immunohistochemistry-Paraffin: DjC9 Antibody [NBP2-33997] - Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
View 1 more images

Applications for DjC9 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DjC9

DjC9 (DnaJ subfamily C member 9) belongs to the Hsp40 (heat shock protein 40) family of proteins that function as co-chaperones to Hsp70.Hsp40 proteins are classified into 3 main subfamilies (A, B, and C). The families are defined by the presence of particular domains which include a highly conserved alpha-helical N-terminal domain termed the J-domain, a glycine/phenylalanine-rich region, a cysteine-rich region, and a C-terminal beta-sheet containing domain. DjC9 is part of subfamily C that bears only the J-domain. DjC9 has been shown to interact with HSP70 through its J domain and activate its ATPase activity. DjC9 is also known as DnaJ protein SB73, HDJC9,DNAJC9,JDD1, and KIAA0974.

Alternate Names

DnaJ (Hsp40) homolog, subfamily C, member 9, dnaJ homolog subfamily C member 9

Gene Symbol

DNAJC9

UniProt

Q8WXX5 (Human)

Additional DjC9 Products

Product Documents for DjC9 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DjC9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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