DLG1 Antibody (JE39-74)

Western Blot: DLG1 Antibody (JE39-74) [NBP3-32929]

Western Blot: DLG1 Antibody (JE39-74) [NBP3-32929] - Western blot analysis of DLG1 on different lysates with Rabbit anti-DLG1 antibody (NBP3-32929) at 1/1,000 dilution.

Lane 1: MCF7 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: U-87 MG cell lysate
Lane 4: SiHa cell lysate
Lane 5: NCI-H226 cell lysate
Lane 6: NIH/3T3 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 100 kDa
Observed band size: 120 kDa

Exposure time: 1 minute 55 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32929) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: DLG1 Antibody (JE39-74) [NBP3-32929]

Immunohistochemistry: DLG1 Antibody (JE39-74) [NBP3-32929] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-DLG1 antibody (NBP3-32929) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32929) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: DLG1 Antibody (JE39-74) [NBP3-32929]

Flow Cytometry: DLG1 Antibody (JE39-74) [NBP3-32929] - Flow cytometric analysis of SiHa cells labeling DLG1.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32929, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).