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DLST Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31715

Novus Biologicals, part of Bio-Techne
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NBP1-31715

Key Product Details

Species Reactivity

Validated:

Human, Rat, Zebrafish

Predicted:

Xenopus (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human DLST. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (87%), Xenopus tropicalis (89%).

Localization

Mitochondrion

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DLST Antibody

Western Blot: DLST Antibody [NBP1-31715]

Western Blot: DLST Antibody [NBP1-31715]

Western Blot: DLST Antibody [NBP1-31715] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLST antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: DLST Antibody [NBP1-31715]

Immunocytochemistry/ Immunofluorescence: DLST Antibody [NBP1-31715]

Immunocytochemistry/Immunofluorescence: DLST Antibody [NBP1-31715] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: DLST Antibody [NBP1-31715]

Immunohistochemistry-Paraffin: DLST Antibody [NBP1-31715]

Immunohistochemistry-Paraffin: DLST Antibody [NBP1-31715] - RT2 xenograft, using DLST antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for DLST Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DLST

DLST - dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex)

Alternate Names

2-oxoglutarate dehydrogenase complex component E2, dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutaratecomplex), Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenasecomplex, dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutaratedehydrogenase complex, mitochondrial, DLTS, E2K, EC 2.3.1, EC 2.3.1.61, OGDC-E2

Gene Symbol

DLST

Additional DLST Products

Product Documents for DLST Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DLST Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.