Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2H12/4

Concentration

0.2 mg/ml

View all DMC1 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human DMC1 protein. (Uniprot: Q14565)

Localization

Nucleus. Chromosome.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08832)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for DMC1 Antibody (2H12/4)

Immunohistochemistry-Paraffin: DMC1 Antibody (2H12/4) [NBP3-07507]

Immunohistochemistry-Paraffin: DMC1 Antibody (2H12/4) [NBP3-07507]

Immunohistochemistry-Paraffin: DMC1 Antibody (2H12/4) [NBP3-07507] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with DMC1 Mouse Monoclonal Antibody (2H12/4).
Immunohistochemistry-Paraffin: DMC1 Antibody (2H12/4) [NBP3-07507]

Immunohistochemistry-Paraffin: DMC1 Antibody (2H12/4) [NBP3-07507]

Immunohistochemistry-Paraffin: DMC1 Antibody (2H12/4) [NBP3-07507] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with DMC1 Mouse Monoclonal Antibody (2H12/4).
Immunohistochemistry-Paraffin: DMC1 Antibody (2H12/4) [NBP3-07507]

Immunohistochemistry-Paraffin: DMC1 Antibody (2H12/4) [NBP3-07507]

Immunohistochemistry-Paraffin: DMC1 Antibody (2H12/4) [NBP3-07507] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with DMC1 Mouse Monoclonal Antibody (2H12/4).

Applications for DMC1 Antibody (2H12/4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: DMC1

DMC-1 is a meiosis-specific homologue of RecA/Rad51 and is an essential component of the meiotic recombination machinery in yeast and higher eukaryotes. DMC-1 is the human equivalent of e. coli recA.

Alternate Names

disrupted meiotic cDNA1, yeast, homolog of, DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologousrecombination (yeast), DMC1 homologue, HsLim15, LIM15yeast homolog) meiosis-specific homologousrecombination, meiotic recombination protein DMC1/LIM15 homolog, MGC150472, MGC150473

Gene Symbol

DMC1

UniProt

Q14565 (Human)

Additional DMC1 Products

Product Documents for DMC1 Antibody (2H12/4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DMC1 Antibody (2H12/4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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