Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1G10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all DMRT1 Antibodies »

Product Summary for DMRT1 Antibody (OTI1G10) - Azide and BSA Free

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-210 of human DMRT1(NP_068770) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

39.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DMRT1 Antibody (OTI1G10) - Azide and BSA Free

Western Blot: DMRT1 Antibody (OTI1G10)Azide and BSA Free [NBP2-70582]

Western Blot: DMRT1 Antibody (OTI1G10)Azide and BSA Free [NBP2-70582]

Western Blot: DMRT1 Antibody (OTI1G10) - Azide and BSA Free [NBP2-70582] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DMRT1 .
Western Blot: DMRT1 Antibody (OTI1G10)Azide and BSA Free [NBP2-70582]

Western Blot: DMRT1 Antibody (OTI1G10)Azide and BSA Free [NBP2-70582]

Western Blot: DMRT1 Antibody (OTI1G10) - Azide and BSA Free [NBP2-70582] - Analysis of extracts (10ug) from 2 different cell lines.

Applications for DMRT1 Antibody (OTI1G10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DMRT1

DMRT1 is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes. This gene exhibits a gonad-specific and sexually dimorphic expression pattern. Defective testicular development and XY feminization occur when this gene is hemizygous.

Alternate Names

DM domain expressed in testis 1, DMT1DM domain expressed in testis protein 1, doublesex and mab-3 related transcription factor 1, doublesex- and mab-3-related transcription factor 1

Gene Symbol

DMRT1

Additional DMRT1 Products

Product Documents for DMRT1 Antibody (OTI1G10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DMRT1 Antibody (OTI1G10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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