DNA Ligase I Antibody (0D6M4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33540
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 0D6M4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-91 of human DNA Ligase I (P18858).
Sequence:
MQRSIMSFFHPKKEGKAKKPEKEASNSSRETEPPPKAALKEWNGVVSESDSPVKRPGRKAARVLGSEGEEEDEALSPAKGQKPALDCSQVS
Sequence:
MQRSIMSFFHPKKEGKAKKPEKEASNSSRETEPPPKAALKEWNGVVSESDSPVKRPGRKAARVLGSEGEEEDEALSPAKGQKPALDCSQVS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DNA Ligase I Antibody (0D6M4)
Immunohistochemistry: DNA Ligase I Antibody (0D6M4) [NBP3-33540] -
Immunohistochemistry: DNA Ligase I Antibody (0D6M4) [NBP3-33540] - Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using DNA Ligase I Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry: DNA Ligase I Antibody (0D6M4) [NBP3-33540] -
Immunohistochemistry: DNA Ligase I Antibody (0D6M4) [NBP3-33540] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using DNA Ligase I Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry: DNA Ligase I Antibody (0D6M4) [NBP3-33540] -
Immunohistochemistry: DNA Ligase I Antibody (0D6M4) [NBP3-33540] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using DNA Ligase I Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Applications for DNA Ligase I Antibody (0D6M4)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: DNA Ligase I
Long Name
DNA ligase 1
Alternate Names
EC 6.5.1.1
Gene Symbol
LIG1
Additional DNA Ligase I Products
Product Documents for DNA Ligase I Antibody (0D6M4)
Product Specific Notices for DNA Ligase I Antibody (0D6M4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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