DNA Ligase I Antibody (7B2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26168
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7B2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human DNA Ligase I [UniProt P18858]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DNA Ligase I Antibody (7B2)
Immunohistochemistry: DNA Ligase I Antibody (7B2) [NBP3-26168] -
Immunohistochemistry: DNA Ligase I Antibody (7B2) [NBP3-26168] - Image of DNA Ligase I Antibody (7B2) diluted at 1:100 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: DNA Ligase I Antibody (7B2) [NBP3-26168] -
Immunohistochemistry: DNA Ligase I Antibody (7B2) [NBP3-26168] - Image of DNA Ligase I Antibody (7B2) diluted at 1:100 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: DNA Ligase I Antibody (7B2) [NBP3-26168] -
Western Blot: DNA Ligase I Antibody (7B2) [NBP3-26168] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate.All lanes: DNA Ligase I Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 102, 89, 99 kDa
Observed band size: 140 kDa
Applications for DNA Ligase I Antibody (7B2)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: DNA Ligase I
Long Name
DNA ligase 1
Alternate Names
EC 6.5.1.1
Gene Symbol
LIG1
Additional DNA Ligase I Products
Product Documents for DNA Ligase I Antibody (7B2)
Product Specific Notices for DNA Ligase I Antibody (7B2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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