DNA Ligase III Antibody (1F3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-41190
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Chicken
Cited:
Human
Applications
Validated:
Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay, Western Blot
Cited:
Block/Neutralize, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1F3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant full-length human DNA Ligase III-b.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Localization
Nuclear and Mitochondrial
Specificity
This antibody will recognize human DNA ligase III.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
113 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DNA Ligase III Antibody (1F3)
![Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190]](https://resources.bio-techne.com/images/products/DNA-Ligase-III-Antibody-1F3-Western-Blot-NBP1-41190-img0003.jpg "Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190]")
Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190]
Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190] - Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.![Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190]](https://resources.bio-techne.com/images/products/DNA-Ligase-III-Antibody-1F3-Western-Blot-NBP1-41190-img0002.jpg "Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190]")
Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190]
Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190] - A. 293T whole cell lysate/extract. B. A431 whole cell lysate/extract. C. HeLa whole cell lysate/extract. D. HepG2 whole cell lysate/extract. DNA ligase III dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.Applications for DNA Ligase III Antibody (1F3)
Application
Recommended Usage
Block/Neutralize
Assay dependent
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunoprecipitation
Assay dependent
Proximity Ligation Assay
Assay dependent
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DNA Ligase III
Additional DNA Ligase III Products
Product Documents for DNA Ligase III Antibody (1F3)
Product Specific Notices for DNA Ligase III Antibody (1F3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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