DNA Ligase III Antibody (HL2280) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25441
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2280
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for DNA Ligase III Antibody (HL2280) - Azide and BSA Free
Immunogen
Recombinant fragment of human DNA Ligase III.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DNA Ligase III Antibody (HL2280) - Azide and BSA Free
Western Blot: DNA Ligase III Antibody (HL2280) - Azide and BSA Free [NBP3-25441] -
Western Blot: DNA Ligase III Antibody (HL2280) - Azide and BSA Free [NBP3-25441] - HepG2 whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody [HL2280] (NBP3-25441) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: DNA Ligase III Antibody (HL2280) - Azide and BSA Free [NBP3-25441] -
Immunohistochemistry-Paraffin: DNA Ligase III Antibody (HL2280) - Azide and BSA Free [NBP3-25441] - DNA ligase III antibody [HL2280] detects DNA ligase III protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. DNA ligase III stained by DNA ligase III antibody [HL2280] (NBP3-25441) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/Immunofluorescence: DNA Ligase III Antibody (HL2280) - Azide and BSA Free [NBP3-25441] -
Immunocytochemistry/Immunofluorescence: DNA Ligase III Antibody (HL2280) - Azide and BSA Free [NBP3-25441] - DNA ligase III antibody [HL2280] detects DNA ligase III protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DNA ligase III stained by DNA ligase III antibody [HL2280] (NBP3-25441) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Applications for DNA Ligase III Antibody (HL2280) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DNA Ligase III
Long Name
DNA ligase 3
Alternate Names
LIG3
Gene Symbol
LIG3
Additional DNA Ligase III Products
Product Documents for DNA Ligase III Antibody (HL2280) - Azide and BSA Free
Product Specific Notices for DNA Ligase III Antibody (HL2280) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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