DNA Ligase III Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92719

Novus Biologicals, part of Bio-Techne
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NBP2-92719-0.02ml
NBP2-92719-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DNA Ligase III Antibodies »

Product Summary for DNA Ligase III Antibody - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 254-460 of human DNA Ligase III (NP_039269.2). CDPRHKDCLLREFRKLCAMVADNPSYNTKTQIIQDFLRKGSAGDGFHGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDEFLLRLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNAQE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

113 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DNA Ligase III Antibody - Azide and BSA Free

Western Blot: DNA Ligase III AntibodyAzide and BSA Free [NBP2-92719]

Western Blot: DNA Ligase III AntibodyAzide and BSA Free [NBP2-92719]

Western Blot: DNA Ligase III Antibody [NBP2-92719] - Analysis of extracts of various cell lines, using at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: DNA Ligase III Antibody - Azide and BSA Free [NBP2-92719]

Immunocytochemistry/ Immunofluorescence: DNA Ligase III Antibody - Azide and BSA Free [NBP2-92719]

Immunocytochemistry/Immunofluorescence: DNA Ligase III Antibody [NBP2-92719] - Analysis of PC-12 cells using LIG3 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: DNA Ligase III Antibody - Azide and BSA Free [NBP2-92719]

Immunohistochemistry-Paraffin: DNA Ligase III Antibody - Azide and BSA Free [NBP2-92719]

Immunohistochemistry-Paraffin: DNA Ligase III Antibody [NBP2-92719] - Immunohistochemistry of paraffin-embedded human colon using DNA Ligase III Rabbit pAb (NBP2-92719) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for DNA Ligase III Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DNA Ligase III

DNA Ligase III is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

DNA ligase 3

Alternate Names

LIG3

Gene Symbol

LIG3

Additional DNA Ligase III Products

Product Documents for DNA Ligase III Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DNA Ligase III Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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