DNA Ligase IV Antibody (2T6J2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15379

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15379-100ul
NBP3-15379-20ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2T6J2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DNA Ligase IV Antibodies »

Product Summary for DNA Ligase IV Antibody (2T6J2)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 504-685 of human DNA Ligase IV (P49917). SRVGSGCTMKELYDLGLKLAKYWKPFHRKAPPSSILCGTEKPEVYIEPCNSVIVQIKAAEIVPSDMYKTGCTLRFPRIEKIRDDKEWHECMTLDDLEQLRGKASGKLASKHLYIGGDDEPQEKKRKAAPKMKKVIGIIEHLKAPNLTNVNKISNIFEDVEFCVMSGTDSQPKPDLENRIAEF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNA Ligase IV Antibody (2T6J2)

Western Blot: DNA Ligase IV Antibody (2T6J2) [NBP3-15379]

Western Blot: DNA Ligase IV Antibody (2T6J2) [NBP3-15379]

Western Blot: DNA Ligase IV Antibody (2T6J2) [NBP3-15379] - Western blot analysis of extracts of various cell lines, using DNA Ligase IV Rabbit mAb (NBP3-15379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for DNA Ligase IV Antibody (2T6J2)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DNA Ligase IV

LIG4 encodes a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome.

Long Name

DNA ligase 4

Alternate Names

LIG4

Gene Symbol

LIG4

Additional DNA Ligase IV Products

Product Documents for DNA Ligase IV Antibody (2T6J2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DNA Ligase IV Antibody (2T6J2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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