DNA Ligase IV Antibody

Name: DNA Ligase IV Antibody
Brand: Novus Biologicals
SKU: NBP2-16182

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16182

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Citations (2)

Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DNA Ligase IV Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DNA Ligase IV. The exact sequence is proprietary.

Reactivity Notes

Sheep (83%), Bovine (86%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DNA Ligase IV Antibody

Western Blot: DNA Ligase IV Antibody [NBP2-16182]

Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.

Immunocytochemistry/ Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182]

Immunocytochemistry/Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182]

Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] - Human ovarian cancer, using DNA ligase IV antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: DNA Ligase IV Antibody [NBP2-16182]

Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.

Western Blot: DNA Ligase IV Antibody [NBP2-16182]

Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.

Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] -

Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] -Rat colon.DNA ligase IV stained by DNA ligase IV antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] -

Immunocytochemistry/Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] -MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DNA ligase IV stained by DNA ligase IV antibody diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] -

Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] - DNA Ligase IV antibody detects DNA Ligase IV protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
DNA Ligase IV stained by DNA Ligase IV antibody (NBP2-16182) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] -

Immunocytochemistry/ Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] - DNA Ligase IV antibody detects DNA Ligase IV protein at nucleus and nucleolus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNA Ligase IV stained by DNA Ligase IV antibody (NBP2-16182) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI .

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Applications for DNA Ligase IV Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DNA Ligase IV

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]

Long Name

DNA ligase 4

Alternate Names

LIG4

Gene Symbol

LIG4

UniProt

P49917 (Human)

Additional DNA Ligase IV Products

Product Documents for DNA Ligase IV Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for DNA Ligase IV Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.

DNA Ligase IV Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16182

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all DNA Ligase IV Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Localization

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for DNA Ligase IV Antibody

Western Blot: DNA Ligase IV Antibody [NBP2-16182]

Immunocytochemistry/ Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182]

Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182]

Western Blot: DNA Ligase IV Antibody [NBP2-16182]

Western Blot: DNA Ligase IV Antibody [NBP2-16182]

Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] -

Immunocytochemistry/Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] -

Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] -

Immunocytochemistry/ Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] -

Applications for DNA Ligase IV Antibody

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: DNA Ligase IV

Long Name

Alternate Names

Gene Symbol

UniProt

Additional DNA Ligase IV Products

Product Documents for DNA Ligase IV Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for DNA Ligase IV Antibody