DNA Ligase IV Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68729

Novus Biologicals, part of Bio-Techne
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NBP2-68729
NBP2-68729-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DNA Ligase IV Antibodies »

Product Summary for DNA Ligase IV Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PQLERERMAYGIKETMLAKLYIELLNLPRDGKDALKLLNYRTPTGTHGDAGDFAMIAYFVLKPRCLQKGSLTIQQVNDLLDSIASNNSAKRKDLIKKSLLQLITQSSALEQKWLIRMIIKDLKLGVSQQTIFSVFHNDAAELHNV

Reactivity Notes

Rat 88%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNA Ligase IV Antibody

Immunocytochemistry/ Immunofluorescence: DNA Ligase IV Antibody [NBP2-68729]

Immunocytochemistry/ Immunofluorescence: DNA Ligase IV Antibody [NBP2-68729]

Immunocytochemistry/Immunofluorescence: DNA Ligase IV Antibody [NBP2-68729] - Staining of human cell line A-431 shows localization to nucleoplasm & cytoplasmic bodies.

Applications for DNA Ligase IV Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
Recommended conditions: Fixation/Permeabilization: PFA/Triton X-100
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNA Ligase IV

LIG4 encodes a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome.

Long Name

DNA ligase 4

Alternate Names

LIG4

Gene Symbol

LIG4

Additional DNA Ligase IV Products

Product Documents for DNA Ligase IV Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DNA Ligase IV Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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