DNA polymerase alpha Antibody (3C11)
Novus Biologicals, part of Bio-Techne | Catalog # H00005422-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3C11
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
POLA (NP_058633, 1363 a.a. ~ 1462 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QFSRTGPLCPACMKATLQPEYSDKSLYTQLCFYRYIFDAECALEKLTTDHEKDKLKKQFFTPKVLQDYRKLKNTAEQFLSRSGYSEVNLSKLFAGCAVKS
Specificity
POLA - polymerase (DNA directed), alpha
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for DNA polymerase alpha Antibody (3C11)
![Western Blot: DNA polymerase alpha Antibody (3C11) [H00005422-M01]](https://resources.bio-techne.com/images/products/DNA-polymerase-alpha-Antibody-3C11-Western-Blot-H00005422-M01-img0004.jpg "Western Blot: DNA polymerase alpha Antibody (3C11) [H00005422-M01]")
Western Blot: DNA polymerase alpha Antibody (3C11) [H00005422-M01]
Western Blot: DNA polymerase alpha Antibody (3C11) [H00005422-M01] - POLA monoclonal antibody (M01), clone 3C11 Analysis of POLA expression in Hela S3 NE.
Sandwich ELISA: DNA polymerase alpha Antibody (3C11) [H00005422-M01] - Detection limit for recombinant GST tagged POLA is approximately 0.3ng/ml as a capture antibody.
Applications for DNA polymerase alpha Antibody (3C11)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: DNA polymerase alpha
Long Name
DNA polymerase alpha catalytic subunit
Alternate Names
EC 2.7.7.7, p180, POLA, POLA1
Entrez Gene IDs
5422 (Human)
Gene Symbol
POLA1
OMIM
312040 (Human)
UniProt
Additional DNA polymerase alpha Products
Product Documents for DNA polymerase alpha Antibody (3C11)
Product Specific Notices for DNA polymerase alpha Antibody (3C11)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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