DNA polymerase alpha Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47256
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 775 and 825 of human Polymerase (DNA directed), Alpha 1, Catalytic Subunit using the numbering given in entry NP_058633.2 (GeneID 5422).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
166 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DNA polymerase alpha Antibody
Western Blot: DNA polymerase alpha Antibody [NBP1-47256]
Western Blot: DNA polymerase alpha Antibody [NBP1-47256] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PolA1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PolA1 was also immunoprecipitated by rabbit anti-PolA1 antibody NBP1-47257, which recognizes a downstream epitopeImmunocytochemistry/ Immunofluorescence: DNA polymerase alpha Antibody [NBP1-47256]
Immunocytochemistry/Immunofluorescence: DNA polymerase alpha Antibody [NBP1-47256] - Analysis of DNA polymerase alpha in mouse A9 fibroblasts that were either Mock infected or were infected with the parvovirus MVM (Minute Virus of Mice). Image courtesy of Dr. Matt Fuller at University of Missouri-Columbia.Applications for DNA polymerase alpha Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Validated from a verified customer review
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Reviewed Applications
Read 1 review rated 5 using NBP1-47256 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: DNA polymerase alpha
Long Name
DNA polymerase alpha catalytic subunit
Alternate Names
EC 2.7.7.7, p180, POLA, POLA1
Gene Symbol
POLA1
UniProt
Additional DNA polymerase alpha Products
Product Documents for DNA polymerase alpha Antibody
Product Specific Notices for DNA polymerase alpha Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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