DNA polymerase alpha Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47257
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 1175 and 1225 of human Polymerase (DNA directed), Alpha 1, Catalytic Subunit using the numbering given in entry NP_058633.2 (GeneID 5422).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DNA polymerase alpha Antibody
Western Blot: DNA polymerase alpha Antibody [NBP1-47257]
Western Blot: DNA polymerase alpha Antibody [NBP1-47257] - Human B cell lysate (40 ug). KSHV infection protects DNA polA1 from hypoxia mediated degradation. Mock or KSHV infected B cells were grown under normoxic or hypoxic condition followed by detecting DNA polA1 levels. Western blot image submitted by a verified customer review.Western Blot: DNA polymerase alpha Antibody [NBP1-47257]
Western Blot: DNA polymerase alpha Antibody [NBP1-47257] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PolA1 antibody NBP1-47257 used for WB at 0.04 ug/mL (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. PolA1 was also immunoprecipitated by rabbit anti-PolA1 antibody NBP1-47256, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).Applications for DNA polymerase alpha Antibody
Application
Recommended Usage
Immunoprecipitation
2 - 5 ug/mg lysate
Western Blot
1:2000 - 1:10000
Reviewed Applications
Read 1 review rated 5 using NBP1-47257 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS, 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: DNA polymerase alpha
Long Name
DNA polymerase alpha catalytic subunit
Alternate Names
EC 2.7.7.7, p180, POLA, POLA1
Entrez Gene IDs
5422 (Human)
Gene Symbol
POLA1
UniProt
Additional DNA polymerase alpha Products
Product Documents for DNA polymerase alpha Antibody
Product Specific Notices for DNA polymerase alpha Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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