DNA polymerase alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82449

Novus Biologicals, part of Bio-Techne
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NBP1-82449
NBP1-82449-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DNA polymerase alpha Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VDLEPMAAKAWDKESEPAEEVKQEADSGKGTVSYLGSFLPDVSCWDIDQEGDSSFSVQEVQVDSSHLPLVKGADEEQVFHFYWLDAYEDQYNQPGVVFLFGKVWIESAETHVSCCVMVK

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24201379)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNA polymerase alpha Antibody

Immunohistochemistry-Paraffin: DNA polymerase alpha Antibody [NBP1-82449]

Immunohistochemistry-Paraffin: DNA polymerase alpha Antibody [NBP1-82449]

Immunohistochemistry-Paraffin: DNA polymerase alpha Antibody [NBP1-82449] - Staining in human lymph node and liver tissues using anti-POLA1 antibody. Corresponding POLA1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: DNA polymerase alpha Antibody [NBP1-82449]

Immunocytochemistry/ Immunofluorescence: DNA polymerase alpha Antibody [NBP1-82449]

Immunocytochemistry/Immunofluorescence: DNA polymerase alpha Antibody [NBP1-82449] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: DNA polymerase alpha Antibody [NBP1-82449]

Immunohistochemistry-Paraffin: DNA polymerase alpha Antibody [NBP1-82449]

Immunohistochemistry-Paraffin: DNA polymerase alpha Antibody [NBP1-82449] - Staining of human liver shows low expression as expected.
View 1 more images

Applications for DNA polymerase alpha Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNA polymerase alpha

DNA polymerase alpha plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p

Long Name

DNA polymerase alpha catalytic subunit

Alternate Names

EC 2.7.7.7, p180, POLA, POLA1

Gene Symbol

POLA1

Additional DNA polymerase alpha Products

Product Documents for DNA polymerase alpha Antibody

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DNA polymerase alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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