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DNA Polymerase iota Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92404

Novus Biologicals, part of Bio-Techne
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NBP2-92404-0.02ml
NBP2-92404-0.1ml

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 50-350 of human POLI (NP_009126.2). ASSRVIVHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEDLTRYREMSYKVTELLEEFSPVVERLGFDENFVDLTEMVEKRLQQLQSDELSAVTVSGHVYNNQSINLLDVLHIRLLVGSQIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGISVAQRIQKLSFGEDNSPVILSGPPQSFSEEDSFKKCSSEVEAKNKIEELL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DNA Polymerase iota Antibody - BSA Free

Western Blot: DNA Polymerase iota AntibodyBSA Free [NBP2-92404]

Western Blot: DNA Polymerase iota AntibodyBSA Free [NBP2-92404]

Western Blot: DNA Polymerase iota Antibody [NBP2-92404] - Analysis of extracts of various cell lines, using DNA Polymerase iota at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Applications for DNA Polymerase iota Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DNA Polymerase iota

DNA polymerase Iota (POLI), also known as Rad30B is a novel human polymerase, unlike any others known. It is an extremely low-fidelity enzyme that is highly error-prone when replicating undamaged and damaged DNA in vitro. To date there is, however, no human disease that can be attributed to overexpression or defects in DNA Pol I.

DNA Pol I antibodies are useful for DNA replication and repair studies and in research on mitosis and cell replication.

Alternate Names

DNA polymerase iota, EC 2.7.7.7, eta2, polymerase (DNA directed) iota, RAD30 homolog B, RAD30Bpolymerase (DNA-directed), iota, RAD3OB

Gene Symbol

POLI

Additional DNA Polymerase iota Products

Product Documents for DNA Polymerase iota Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DNA Polymerase iota Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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