DNA polymerase sigma Antibody (2F8)
Novus Biologicals, part of Bio-Techne | Catalog # H00011044-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 2F8
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
POLS (NP_008930, 2 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SPCPEEAAMRREVVKRIETVVKDLWPTADVQIFGSFSTGLYLPTSDIDLVVFGKWERPPLQLLEQALRKHNVAEPCSIKVLDKATVPIIKLTDQETEVKVDISFNMETG
Specificity
POLS - polymerase (DNA directed) sigma
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for DNA polymerase sigma Antibody (2F8)
![Western Blot: DNA polymerase sigma Antibody (2F8) [H00011044-M01]](https://resources.bio-techne.com/images/products/DNA-polymerase-sigma-Antibody-2F8-Western-Blot-H00011044-M01-img0004.jpg "Western Blot: DNA polymerase sigma Antibody (2F8) [H00011044-M01]")
Western Blot: DNA polymerase sigma Antibody (2F8) [H00011044-M01]
Western Blot: DNA polymerase sigma Antibody (2F8) [H00011044-M01] - Analysis of POLS over-expressed 293 cell line, cotransfected with POLS Validated Chimera RNAi ( Cat # H00011044-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with POLS monoclonal antibody (M01), clone 2F8 (Cat # H00011044-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.![Western Blot: DNA polymerase sigma Antibody (2F8) [H00011044-M01]](https://resources.bio-techne.com/images/products/DNA-polymerase-sigma-Antibody-2F8-Western-Blot-H00011044-M01-img0001.jpg "Western Blot: DNA polymerase sigma Antibody (2F8) [H00011044-M01]")
Western Blot: DNA polymerase sigma Antibody (2F8) [H00011044-M01]
Western Blot: DNA polymerase sigma Antibody (2F8) [H00011044-M01] - Analysis of POLS expression in transfected 293T cell line by POLS monoclonal antibody (M01), clone 2F8. Lane 1: POLS transfected lysate (59.9 KDa). Lane 2: Non-transfected lysate.![ELISA: DNA polymerase sigma Antibody (2F8) [H00011044-M01]](https://resources.bio-techne.com/images/products/DNA-polymerase-sigma-Antibody-2F8-ELISA-H00011044-M01-img0006.jpg "ELISA: DNA polymerase sigma Antibody (2F8) [H00011044-M01]")
ELISA: DNA polymerase sigma Antibody (2F8) [H00011044-M01]
ELISA: DNA polymerase sigma Antibody (2F8) [H00011044-M01] - Detection limit for recombinant GST tagged POLS is approximately 0.3ng/ml as a capture antibody.Applications for DNA polymerase sigma Antibody (2F8)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: DNA polymerase sigma
Alternate Names
DNA polymerase kappa, DNA polymerase sigma, EC 2.7.7.7, LAK-1TUTase 5, PAP associated domain containing 7, POLKTRF41, POLSTUTASE5, polymerase (DNA directed) sigma, polymerase (DNA-directed) sigma, Terminal uridylyltransferase 5, Topoisomerase-related function protein 4-1, TRF4-1LAK1, TRF4PAP-associated domain-containing protein 7
Entrez Gene IDs
11044 (Human)
Gene Symbol
TENT4A
OMIM
605198 (Human)
UniProt
Additional DNA polymerase sigma Products
Product Documents for DNA polymerase sigma Antibody (2F8)
Product Specific Notices for DNA polymerase sigma Antibody (2F8)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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