DNA polymerase sigma Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56356
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was generated by immunizing rabbits with a synthetic peptide containing amino acids 447-462 (GTASLKAVHHMSSPAI) of human TRF4-1, GenBank no NP_008930.1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DNA polymerase sigma Antibody
Western Blot: DNA polymerase sigma Antibody [NB100-56356]
Western Blot: DNA polymerase sigma Antibody [NB100-56356] - Analysis of DNA polymerase sigma in HeLa cell lysate using NB100-56356 at A) 2 ug/ml and B) 5 ug/ml.Immunocytochemistry/ Immunofluorescence: DNA polymerase sigma Antibody [NB100-56356]
Immunocytochemistry/Immunofluorescence: DNA polymerase sigma Antibody [NB100-56356] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-DNA polymerase sigma Antibody NB100-56356 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Cells were imaged using a 100X objective and digitally deconvolved.Applications for DNA polymerase sigma Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 - 1:500
Western Blot
1:500 - 1:1000
Application Notes
Various isoforms of TRF4-1 with different amino acid (aa) sizes, including 541, 517, 360 aa have been described.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS with 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DNA polymerase sigma
Alternate Names
DNA polymerase kappa, DNA polymerase sigma, EC 2.7.7.7, LAK-1TUTase 5, PAP associated domain containing 7, POLKTRF41, POLSTUTASE5, polymerase (DNA directed) sigma, polymerase (DNA-directed) sigma, Terminal uridylyltransferase 5, Topoisomerase-related function protein 4-1, TRF4-1LAK1, TRF4PAP-associated domain-containing protein 7
Entrez Gene IDs
11044 (Human)
Gene Symbol
TENT4A
UniProt
Additional DNA polymerase sigma Products
Product Documents for DNA polymerase sigma Antibody
Product Specific Notices for DNA polymerase sigma Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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