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DNA polymerase sigma Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56356

Novus Biologicals, part of Bio-Techne
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NB100-56356
NB100-56356SS

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was generated by immunizing rabbits with a synthetic peptide containing amino acids 447-462 (GTASLKAVHHMSSPAI) of human TRF4-1, GenBank no NP_008930.1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNA polymerase sigma Antibody

Western Blot: DNA polymerase sigma Antibody [NB100-56356]

Western Blot: DNA polymerase sigma Antibody [NB100-56356]

Western Blot: DNA polymerase sigma Antibody [NB100-56356] - Analysis of DNA polymerase sigma in HeLa cell lysate using NB100-56356 at A) 2 ug/ml and B) 5 ug/ml.
Immunocytochemistry/ Immunofluorescence: DNA polymerase sigma Antibody [NB100-56356]

Immunocytochemistry/ Immunofluorescence: DNA polymerase sigma Antibody [NB100-56356]

Immunocytochemistry/Immunofluorescence: DNA polymerase sigma Antibody [NB100-56356] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-DNA polymerase sigma Antibody NB100-56356 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Cells were imaged using a 100X objective and digitally deconvolved.

Applications for DNA polymerase sigma Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Western Blot

1:500 - 1:1000
Application Notes
Various isoforms of TRF4-1 with different amino acid (aa) sizes, including 541, 517, 360 aa have been described.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS with 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNA polymerase sigma

TRF4-1 is a nuclear protein whose expression is cell cycle-regulated at a post-transcriptional level. Two genes, TRF4-1 and TRF4-2, belonging to this family have been identified. TRF4 is also required to maintain a different aspect of higher order chromosome structure, sister chromatid cohesion.

Alternate Names

DNA polymerase kappa, DNA polymerase sigma, EC 2.7.7.7, LAK-1TUTase 5, PAP associated domain containing 7, POLKTRF41, POLSTUTASE5, polymerase (DNA directed) sigma, polymerase (DNA-directed) sigma, Terminal uridylyltransferase 5, Topoisomerase-related function protein 4-1, TRF4-1LAK1, TRF4PAP-associated domain-containing protein 7

Entrez Gene IDs

11044 (Human)

Gene Symbol

TENT4A

Additional DNA polymerase sigma Products

Product Documents for DNA polymerase sigma Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DNA polymerase sigma Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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