DNAI1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84466

Novus Biologicals, part of Bio-Techne
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NBP1-84466
NBP1-84466-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DNAI1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: APHKQPHKQSISIGRGTRKRDEDSGTEVGEGTDEWAQSKATVRPPDQLELTDAELKEEFTRILTANNPHAPQNIVRYSFKEGTYKPIGFVNQLAVHYTQV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (80%). Reactivity reported in scientific literature (PMID: 24298994)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNAI1 Antibody

Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466]

Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466]

Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining in human testis and prostate tissues using NBP1-84466 antibody. Corresponding DNAI1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466]

Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466]

Immunohistochemistry-Paraffin: DNAI1 Antibody [NBP1-84466] - Staining of human fallopian tube, placenta, prostate and testis using Anti-DNAI1 antibody NBP1-84466 (A) shows similar protein distribution across tissues to independent antibody NBP1-84465 (B).
Western Blot: DNAI1 Antibody [NBP1-84466]

Western Blot: DNAI1 Antibody [NBP1-84466]

Western Blot: DNAI1 Antibody [NBP1-84466] - Analysis in control (vector only transfected HEK293T lysate) and dNAI1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
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Applications for DNAI1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 25700162). For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNAI1

The inner- and outer-arm dyneins, which bridge between the doublet microtubules in axonemes, are the force-generatingproteins responsible for the sliding movement in axonemes. The intermediate and light chains, thought to form the baseof the dynein arm, help mediate attachment and may also participate in regulating dynein activity. This gene encodesan intermediate chain dynein, belonging to the large family of motor proteins. Mutations in this gene result inabnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia (PCD) and Kartagener syndrome.(provided by RefSeq)

Alternate Names

Axonemal dynein intermediate chain 1, CILD1MGC26204, dynein intermediate chain DNAI1, dynein, axonemal, intermediate chain 1, dynein, axonemal, intermediate polypeptide 1, ICS, ICS1, immotile cilia syndrome 1, PCDdynein intermediate chain 1, axonemal

Gene Symbol

DNAI1

Additional DNAI1 Products

Product Documents for DNAI1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DNAI1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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