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DNAJA2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38373

Novus Biologicals, part of Bio-Techne
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NBP3-38373-100ul
NBP3-38373-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 233-412 of human DNAJA2 (NP_005871.1).

Sequence:
FTGEADQAPGVEPGDIVLLLQEKEHEVFQRDGNDLHMTYKIGLVEALCGFQFTFKHLDGRQIVVKYPPGKVIEPGCVRVVRGEGMPQYRNPFEKGDLYIKFDVQFPENNWINPDKLSELEDLLPSRPEVPNIIGETEEVELQEFDSTRGSGGGQRREAYNDSSDEESSSHHGPGVQCAHQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DNAJA2 Antibody

DNAJA2 Antibody

Western Blot: DNAJA2 Antibody [NBP3-38373] -

Western Blot: DNAJA2 Antibody [NBP3-38373] - Western blot analysis of various lysates using DNAJA2 Rabbit pAb at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
DNAJA2 Antibody

Immunocytochemistry/ Immunofluorescence: DNAJA2 Antibody [NBP3-38373] -

Immunocytochemistry/ Immunofluorescence: DNAJA2 Antibody [NBP3-38373] - Immunofluorescence analysis of C6 cells using DNAJA2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
DNAJA2 Antibody

Immunocytochemistry/ Immunofluorescence: DNAJA2 Antibody [NBP3-38373] -

Immunocytochemistry/ Immunofluorescence: DNAJA2 Antibody [NBP3-38373] - Immunofluorescence analysis of NIH/3T3 cells using DNAJA2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for DNAJA2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DNAJA2

DNAJA2 is encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro.

Alternate Names

cell cycle progression 3 protein, Cell cycle progression restoration gene 3 protein, CPR3PRO3015, DJ3, DJA2, DNAJ, DnaJ (Hsp40) homolog, subfamily A, member 2, dnaJ homolog subfamily A member 2, Dnj3, HIRA interacting protein 4, HIRA-interacting protein 4, HIRIP4RDJ2, Renal carcinoma antigen NY-REN-14

Gene Symbol

DNAJA2

Additional DNAJA2 Products

Product Documents for DNAJA2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DNAJA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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