DNAJB11 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84900

Novus Biologicals, part of Bio-Techne
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NBP1-84900
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DNAJB11 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DSEKRKQYDTYGEEGLKDGHQSSHGDIFSHFFGDFGFMFGGTPRQQDRNIPRGSDIIVDLEVTLEEVYAGNFVEVVRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNAJB11 Antibody

Western Blot: DNAJB11 Antibody [NBP1-84900]

Western Blot: DNAJB11 Antibody [NBP1-84900]

Western Blot: DNAJB11 Antibody [NBP1-84900] - Analysis in control (vector only transfected HEK293T lysate) and DNAJB11 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: DNAJB11 Antibody [NBP1-84900]

Immunohistochemistry-Paraffin: DNAJB11 Antibody [NBP1-84900]

Immunohistochemistry-Paraffin: DNAJB11 Antibody [NBP1-84900] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.
Western Blot: DNAJB11 Antibody [NBP1-84900]

Western Blot: DNAJB11 Antibody [NBP1-84900]

Western Blot: DNAJB11 Antibody [NBP1-84900] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
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Applications for DNAJB11 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNAJB11

Members of the heat shock protein 40 (HSP 40) family of proteins all contain a highly conserved J domain that associates with HSP 70 and regulates the function of HSP 70 by activating its adenosine triphosphatase activity. ERdj3, an HSP 40 chaperone, is expressed in the ER lumen, where it interacts with BiP, a molecule involved in retrotranslocating proteins out of the ER. ERdj3 also associates with several other protein substrates, including unfolded light chains, a nonsecreted Ig light chain mutant and a VSV-G ts045 mutant. Shiga toxin (Stx) is a bacterial tool that enzymatically inactivates the 28S rRNA, inhibiting protein synthesis of infected cells. Stx also interacts with ERdj3 and Sec 61 to form a complex through which proteins are retrotranslocated to the cytoplasm. ERdj3 may play a role in the ER quality control system.

Alternate Names

ABBP-2, APOBEC1-binding protein 2, DnaJ (Hsp40) homolog, subfamily B, member 11, dnaJ homolog subfamily B member 11, DnaJ protein homolog 9, EDJABBP2, ER-associated DNAJ, ER-associated dnaJ protein 3, ER-associated Hsp40 co-chaperone, ERdj3DJ9, ERJ3, ERj3p, hDj9, hDj-9, HEDJERj3, Human DnaJ protein 9, PRO1080, PWP1-interacting protein 4, UNQ537

Gene Symbol

DNAJB11

Additional DNAJB11 Products

Product Documents for DNAJB11 Antibody

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Certificate of Analysis

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Product Specific Notices for DNAJB11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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