DNAJB2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83188

Novus Biologicals, part of Bio-Techne
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NBP1-83188
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DNAJB2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LTGTGTGPSRAEAGSGGPGFTFTFRSPEEVFREFFGSGDPFAELFDDLGPFSELQNRGSRHSGPFFTFSSSFPGHSDFSSSSFSFSPGAGAFRSVS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNAJB2 Antibody

Western Blot: DNAJB2 Antibody [NBP1-83188]

Western Blot: DNAJB2 Antibody [NBP1-83188]

Western Blot: DNAJB2 Antibody [NBP1-83188] - Analysis in control (vector only transfected HEK293T lysate) and DNAJB2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: DNAJB2 Antibody [NBP1-83188]

Immunocytochemistry/ Immunofluorescence: DNAJB2 Antibody [NBP1-83188]

Immunocytochemistry/Immunofluorescence: DNAJB2 Antibody [NBP1-83188] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane.
Immunohistochemistry-Paraffin: DNAJB2 Antibody [NBP1-83188]

Immunohistochemistry-Paraffin: DNAJB2 Antibody [NBP1-83188]

Immunohistochemistry-Paraffin: DNAJB2 Antibody [NBP1-83188] - Staining of human cerebellum shows strong nuclear membrane and cytoplasmic positivity in Purkinje cells.

Applications for DNAJB2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNAJB2

Alternate Names

DnaJ (Hsp40) homolog, subfamily B, member 2, HSJ-1, HSPF3neuronal DNAJ-like 1

Gene Symbol

DNAJB2

Additional DNAJB2 Products

Product Documents for DNAJB2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DNAJB2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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