Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockout Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all DNAJC2 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 500 and 550 of human DnaJ (Hsp40) Homolog, Subfamily C, Member 2 using the numbering given in entry NP_055192.1 (GeneID 27000).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNAJC2 Antibody

Western Blot: DNAJC2 Antibody [NBP2-12802]

Western Blot: DNAJC2 Antibody [NBP2-12802]

Western Blot: DNAJC2 Antibody [NBP2-12802] - Detection of human and mouse MPP11 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MPP11 antibody NBP2-12802 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. MPP11 was also immunoprecipitated by rabbit anti-MPP11 antibody NBP2-12803, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Applications for DNAJC2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID 28402505)

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DNAJC2

DNAJC2 is a member of the M-phase phosphoprotein (MPP) family. The gene encodes a phosphoprotein with a J domain and a Myb DNA-binding domain which localizes to both the nucleus and the cytosol. The protein is capable of forming a heterodimeric complex that associates with ribosomes, acting as a molecular chaperone for nascent polypeptide chains as they exit the ribosome. This protein was identified as a leukemia-associated antigen and expression of the gene is upregulated in leukemic blasts. Also, chromosomal aberrations involving this gene are associated with primary head and neck squamous cell tumors. This gene has a pseudogene on chromosome 6. Alternatively spliced variants which encode different protein isoforms have been described. [provided by RefSeq]

Alternate Names

DnaJ (Hsp40) homolog, subfamily C, member 2, dnaJ homolog subfamily C member 2, MPHOSPH11M-phase phosphoprotein 11, MPP11Zuotin-related factor 1, ZRF1zuotin, ZUO1, zuotin related factor 1

Gene Symbol

DNAJC2

UniProt

Q99543 (Human)

Additional DNAJC2 Products

Product Documents for DNAJC2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DNAJC2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.