DNAJC8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87015

Novus Biologicals, part of Bio-Techne
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NBP1-87015
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DNAJC8 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ELEIKRKEREAKEMHERKRQREEEIEAQEKAKREREWQKNFEESRDGRVDSWRNFQANTKGKKEKKNRTFLRPPKVK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNAJC8 Antibody

Western Blot: DNAJC8 Antibody [NBP1-87015]

Western Blot: DNAJC8 Antibody [NBP1-87015]

Western Blot: DNAJC8 Antibody [NBP1-87015] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: NTERA-2 High
Immunohistochemistry-Paraffin: DNAJC8 Antibody [NBP1-87015]

Immunohistochemistry-Paraffin: DNAJC8 Antibody [NBP1-87015]

Immunohistochemistry-Paraffin: DNAJC8 Antibody [NBP1-87015] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Simple Western: DNAJC8 Antibody [NBP1-87015]

Simple Western: DNAJC8 Antibody [NBP1-87015]

Simple Western: DNAJC8 Antibody [NBP1-87015] - Simple Western lane view shows a specific band for DNAJC8 in 0.2 mg/ml of Ntera-2 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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Applications for DNAJC8 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Simple Western

1:5

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Reviewed Applications

Read 1 review rated 4 using NBP1-87015 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNAJC8

DjC8 (DnaJ subfamily C member 8) belongs to the Hsp40 (heat shock protein 40) family of proteins that function as co-chaperones to Hsp70.Hsp40 proteins are classified into 3 main subfamilies (A, B, and C). The families are defined by the presence of particular domains which include a highly conserved alpha-helical N-terminal domain termed the J-domain, a glycine/phenylalanine-rich region, a cysteine-rich region, and a C-terminal beta-sheet containing domain. Djc8 is part of subfamily C that bears only the J-domain. DjC8 is also known as splicing protein spf31, and DNAJC8.

Alternate Names

DnaJ (Hsp40) homolog, subfamily C, member 8, dnaJ homolog subfamily C member 8, HSPC331, SPF31Splicing protein spf31

Gene Symbol

DNAJC8

Additional DNAJC8 Products

Product Documents for DNAJC8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DNAJC8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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