DNASE1L3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16198
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Rat (90%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human DNASE1L3. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (88%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DNASE1L3 Antibody
![Western Blot: DNASE1L3 Antibody [NBP2-16198]](https://resources.bio-techne.com/images/products/DNASE1L3-Antibody-Western-Blot-NBP2-16198-img0007.jpg "Western Blot: DNASE1L3 Antibody [NBP2-16198]")
Western Blot: DNASE1L3 Antibody [NBP2-16198]
Western Blot: DNASE1L3 Antibody [NBP2-16198] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DNase IL3 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Western Blot: DNASE1L3 Antibody [NBP2-16198]](https://resources.bio-techne.com/images/products/DNASE1L3-Antibody-Western-Blot-NBP2-16198-img0005.jpg "Western Blot: DNASE1L3 Antibody [NBP2-16198]")
Western Blot: DNASE1L3 Antibody [NBP2-16198]
Western Blot: DNASE1L3 Antibody [NBP2-16198] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with DNase IL3 antibody [N3C3] diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: DNASE1L3 Antibody [NBP2-16198] -
Immunocytochemistry/ Immunofluorescence: DNASE1L3 Antibody [NBP2-16198] - DNASE1L3 antibody [N3C3] detects DNASE1L3 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNASE1L3 stained by DNASE1L3 antibody [N3C3] (NBP2-16198) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Applications for DNASE1L3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: DNASE1L3
Alternate Names
deoxyribonuclease gamma, deoxyribonuclease I-like 3Liver and spleen DNase, deoxyribonuclease I-like III, DNAS1L3LSDDHP2, DNase gamma, DNase I homolog protein 2, DNase I homolog protein DHP2, DNase I-like 3, EC 3.1.21.-, EC 3.1.21.1, LS-DNase
Gene Symbol
DNASE1L3
UniProt
Additional DNASE1L3 Products
Product Documents for DNASE1L3 Antibody
Product Specific Notices for DNASE1L3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.