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Doc2-alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16209

Novus Biologicals, part of Bio-Techne
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NBP2-16209

Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (98%), Human (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Doc2-alpha. The exact sequence is proprietary.

Localization

Cytoplasmic vesicle; secretory vesicle; synaptic vesicle membrane; Peripheral membrane protein; Cell junction; synapse; synaptosome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Doc2-alpha Antibody

Western Blot: Doc2-alpha Antibody [NBP2-16209]

Western Blot: Doc2-alpha Antibody [NBP2-16209]

Western Blot: Doc2-alpha Antibody [NBP2-16209] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Doc2-alpha Antibody [NBP2-16209]

Immunocytochemistry/ Immunofluorescence: Doc2-alpha Antibody [NBP2-16209]

Immunocytochemistry/Immunofluorescence: Doc2-alpha Antibody [NBP2-16209] - DOC2A antibody [N2C3] detects DOC2A protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DOC2A stained by DOC2A antibody [N2C3] diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500. Blue: Fluoroshield with DAPI.

Applications for Doc2-alpha Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Doc2 alpha

There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2A is mainly expressed in brain and is suggested to be involved in Ca(2+)-dependent neurotransmitter release. [provided by RefSeq]

Long Name

Double C2-like Domains, alpha

Alternate Names

DOC2A

Gene Symbol

DOC2A

UniProt

Additional Doc2 alpha Products

Product Documents for Doc2-alpha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Doc2-alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.