Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all DOCK7 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 175 and 225 of human Dedicator of Cytokinesis 7 using the numbering given in entry Q96N67.4 (GeneID 85440).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DOCK7 Antibody

Western Blot: DOCK7 Antibody [NBP1-78748]

Western Blot: DOCK7 Antibody [NBP1-78748]

Western Blot: DOCK7 Antibody [NBP1-78748] - A549 human alveolar adenocarcinoma cell line. Total A549 cell extract diluted to 1/4 in Laemmli buffer. Western blot image submitted by a verified customer review.
Western Blot: DOCK7 Antibody [NBP1-78748]

Western Blot: DOCK7 Antibody [NBP1-78748]

Western Blot: DOCK7 Antibody [NBP1-78748] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-DOCK7 antibody used for WB at 0.1 mcg/mL (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. DOCK7 was also immunoprecipitated by rabbit anti-DOCK7 antibody (NBP1-78749) which recognizes a downstream epitope.

Applications for DOCK7 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
DOCK7 antibody validated for WB from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP1-78748 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DOCK7

DOCK7 functions as a guanine nucleotide exchange factor (GEF), which activates Rac1 and Rac3 Rho small GTPases byexchanging bound GDP for free GTP. Does not have a GEF activity for CDC42. Required for STMN1 'Ser-15' phosphorylationduring axon formation and cons

Alternate Names

dedicator of cytokinesis 7, dedicator of cytokinesis protein 7, KIAA1771ZIR2

Gene Symbol

DOCK7

UniProt

Q96N67.4 (Human)

Additional DOCK7 Products

Product Documents for DOCK7 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DOCK7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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