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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # DG1/1485

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant human canine1/TMEM16A protein fragment (around aa 2-101) (exact sequence is proprietary) (Uniprot: Q5XX6)

Localization

Cell Surface and Cytoplasm

Marker

Gastrointestinal Stromal Tumor Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53162).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for DOG1/TMEM16A Antibody (DG1/1485) - Azide and BSA Free

Immunohistochemistry-Paraffin: DOG1/TMEM16A Antibody (DG1/1485) - Azide and BSA Free [NBP2-54485]

Immunohistochemistry-Paraffin: DOG1/TMEM16A Antibody (DG1/1485) - Azide and BSA Free [NBP2-54485]

Immunohistochemistry-Paraffin: DOG1/TMEM16A Antibody (DG1/1485) - Azide and BSA Free [NBP2-54485] - Formalin-fixed, paraffin-embedded human GIST stained with DOG-1 Monoclonal Antibody (DG1/1485).
Protein Array: DOG1/TMEM16A Antibody (DG1/1485) - Azide and BSA Free [NBP2-54485]

Protein Array: DOG1/TMEM16A Antibody (DG1/1485) - Azide and BSA Free [NBP2-54485]

Protein Array: DOG1/TMEM16A Antibody (DG1/1485) - Azide and BSA Free [NBP2-54485] - Analysis of Protein Array containing more than 19,000 full-length human proteins using DOG1/TMEM16A Antibody (DG1/1485). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.

Applications for DOG1/TMEM16A Antibody (DG1/1485) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Protein Array

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: ANO1/TMEM16A

Long Name

Anoctamin 1

Alternate Names

DOG1, ORAOV2, TAOS2, TMEM16A

Gene Symbol

ANO1

Additional ANO1/TMEM16A Products

Product Documents for DOG1/TMEM16A Antibody (DG1/1485) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DOG1/TMEM16A Antibody (DG1/1485) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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