DOK1 Antibody (6U4U9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15713

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15713-100ul
NBP3-15713-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6U4U9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DOK1 Antibodies »

Product Summary for DOK1 Antibody (6U4U9)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 382-481 of human DOK1 (Q99704). EPKDAWWCQARVKEEGYELPYNPATDDYAVPPPRSTKPLLAPKPQGPAFPEPGTATGSGIKSHNSALYSQVQKSGASGSWDCGLSRVGTDKTGVKSEGST

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DOK1 Antibody (6U4U9)

Western Blot: DOK1 Antibody (6U4U9) [NBP3-15713]

Western Blot: DOK1 Antibody (6U4U9) [NBP3-15713]

Western Blot: DOK1 Antibody (6U4U9) [NBP3-15713] - Western blot analysis of extracts of various cell lines, using DOK1 antibody (NBP3-15713) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: DOK1 Antibody (6U4U9) [NBP3-15713]

Immunocytochemistry/ Immunofluorescence: DOK1 Antibody (6U4U9) [NBP3-15713]

Immunocytochemistry/Immunofluorescence: DOK1 Antibody (6U4U9) [NBP3-15713] - Immunofluorescence analysis of BALB-3T3 cells using DOK1 Rabbit mAb (NBP3-15713) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for DOK1 Antibody (6U4U9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DOK1

Dok-1 associates with the Ras GTPase-activating protein (Ras GAP) upon tyrosine phosphorylation. Evidence suggests that Dok-1 (also designated p62dok) is a substrate of the constitutive tyrosine kinase activity of p210 Bcr-Abl, a fusion protein caused by the t(9;22) translocation and associated with chronic myelogenous leukemia. Dok-1, as well as the tyrosine kinase substrates IRS-1 and Cas, are members of a class of "docking" proteins which contain multiple tyrosine residues and putative SH2 binding sites. Dok-1 is suspected to be the substrate phosphorylated in response to stimulation by a number of growth factors, including PDGF, VEGF, insulin and IGF. Dok-2 (also designated p56dok) has also been identified as a potential mediator of the effects of p210 Bcr-Abl.

Alternate Names

docking protein 1, docking protein 1 (downstream of tyrosine kinase 1), docking protein 1, 62kD (downstream of tyrosine kinase 1), docking protein 1, 62kDa (downstream of tyrosine kinase 1), Downstream of tyrosine kinase 1, MGC117395, MGC138860, p62(dok), p62dok, pp62

Gene Symbol

DOK1

Additional DOK1 Products

Product Documents for DOK1 Antibody (6U4U9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DOK1 Antibody (6U4U9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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