Dopamine D1R/DRD1 Antibody (SG2-D1a)
Novus Biologicals, part of Bio-Techne | Catalog # NB110-60017
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse, Rat, Bovine (Negative), Canine (Negative), Human (Negative), Porcine (Negative), Rabbit (Negative), Sheep (Negative)
Cited:
Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # SG2-D1a
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant rat Dopamine Receptor D1 protein near the C-terminus. [Swiss-Prot# P18901]
Localization
Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note: Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.
Specificity
This antibody is specific for Dopamine Receptor D1A. There is no cross-reactivity with D1B (D5) receptor.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for Dopamine D1R/DRD1 Antibody (SG2-D1a)
Western Blot: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017]
Western Blot: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of lysates from Sf9 cells which were transfected with rat DR-D1b (Lane 1) or DR-D1a (Lane 2) using DRD1 antibody (clone SG2-D1a). This clone detected D1a receptor only and was not cross-reactive against DR-D1b (Note: these results are similiar to those shown in J. Neuroimmunol. 101:170-187 publication.)Immunohistochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017]
Immunohistochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Representative images of immunofluorescence for c-fos and dopamine receptor D1 in the NAc. Image collected and cropped by CiteAb from the following publication (nature.com/articles/ncomms11829), licensed under a CC-BY license.Immunohistochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017]
Immunohistochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Dopamine D1 R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of Dopamine Receptor D1 in rat retinal whole mounts and sections. Image from verified customer review.Applications for Dopamine D1R/DRD1 Antibody (SG2-D1a)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 31462765)
Immunohistochemistry
1:1000
Immunohistochemistry-Frozen
1:1000
Western Blot
1:200-1:500
Application Notes
In Western blot multiple bands are seen around 45 and 100 kDa (dimer) in SF9 cell lysates overexpressing D1a and a single band at 90 kDa in striatal tissue lysate. Do NOT boil samples.
Reviewed Applications
Read 3 reviews rated 4.7 using NB110-60017 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Dopamine D1R/DRD1
Long Name
Dopamine D1 Receptor
Alternate Names
DADR, Dopamine D1 R, DRD1
Gene Symbol
DRD1
Additional Dopamine D1R/DRD1 Products
Product Documents for Dopamine D1R/DRD1 Antibody (SG2-D1a)
Product Specific Notices for Dopamine D1R/DRD1 Antibody (SG2-D1a)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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