Dopamine D3R/DRD3 Antibody (JE33-33)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32277
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE33-33
Concentration
1 mg/ml
Product Summary for Dopamine D3R/DRD3 Antibody (JE33-33)
Immunogen
Synthetic peptide within Human Dopamine D3R/DRD3 aa 1-50 / 400. (Uniprot: P35462)
Localization
Cell membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Dopamine D3R/DRD3 Antibody (JE33-33)
Western Blot: Dopamine D3R/DRD3 Antibody (JE33-33) [NBP3-32277]
Western Blot: Dopamine D3R/DRD3 Antibody (JE33-33) [NBP3-32277] - Western blot analysis of Dopamine D3R/DRD3 on different lysates with Rabbit anti-Dopamine D3R/DRD3 antibody (NBP3-32277) at 1/1,000 dilution.Lane 1: HeLa cell lysate (10 ug/Lane)
Lane 2: SH-SY5Y cell lysate (10 ug/Lane)
Lane 3: Mouse brain tissue lysate (20 ug/Lane)
Lane 4: Rat brain tissue lysate (20 ug/Lane)
Predicted band size: 44 kDa
Observed band size: 50 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32277) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for Dopamine D3R/DRD3 Antibody (JE33-33)
Application
Recommended Usage
Western Blot
1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Dopamine D3 R/DRD3
Long Name
Dopamine D3 Receptor
Alternate Names
D3DR, Dopamine D3R, DRD3, ETM1, FET1
Gene Symbol
DRD3
Additional Dopamine D3 R/DRD3 Products
Product Documents for Dopamine D3R/DRD3 Antibody (JE33-33)
Product Specific Notices for Dopamine D3R/DRD3 Antibody (JE33-33)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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