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Doppel Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31928

Novus Biologicals, part of Bio-Techne
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NBP2-31928
NBP2-31928-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Doppel Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GIKHRIKWNRKALPSTAQITEAQVAENRPGAFIKQGRKLDIDFGAEGNRYYEANYWQF

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Doppel Antibody

Immunohistochemistry-Paraffin: Doppel Antibody [NBP2-31928]

Immunohistochemistry-Paraffin: Doppel Antibody [NBP2-31928]

Immunohistochemistry-Paraffin: Doppel Antibody [NBP2-31928] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: Doppel Antibody [NBP2-31928]

Immunohistochemistry-Paraffin: Doppel Antibody [NBP2-31928]

Immunohistochemistry-Paraffin: Doppel Antibody [NBP2-31928] - Staining of human endometrium shows low expression as expected.
Immunohistochemistry-Paraffin: Doppel Antibody [NBP2-31928]

Immunohistochemistry-Paraffin: Doppel Antibody [NBP2-31928]

Immunohistochemistry-Paraffin: Doppel Antibody [NBP2-31928] - Staining in human testis and endometrium tissues using anti-PRND antibody. Corresponding PRND RNA-seq data are presented for the same tissues.

Applications for Doppel Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Doppel

The Doppel gene is found on chromosome 20, approximately 20 kbp downstream of the gene encoding cellular prion protein, to which it is biochemically and structurally similar. The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored g

Alternate Names

dJ1068H6.4, DOPPEL, DPLprion gene complex, downstream, MGC41841, Prion protein 2, prion protein 2 (dublet), PrPLPprion-like protein doppel

Gene Symbol

PRND

UniProt

Additional Doppel Products

Product Documents for Doppel Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Doppel Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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