DPH2 Antibody (OTI4E7) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70585

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4E7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all DPH2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human DPH2(NP_001375) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

51.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DPH2 Antibody (OTI4E7) - Azide and BSA Free

Western Blot: DPH2 Antibody (OTI4E7)Azide and BSA Free [NBP2-70585]

Western Blot: DPH2 Antibody (OTI4E7)Azide and BSA Free [NBP2-70585]

Western Blot: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPH2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPH2.
Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Immunohistochemistry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - Staining of paraffin-embedded Human prostate tissue using anti-DPH2 mouse monoclonal antibody.
Flow Cytometry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Flow Cytometry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585]

Flow Cytometry: DPH2 Antibody (OTI4E7) - Azide and BSA Free [NBP2-70585] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-DPH2 antibody, and then analyzed by flow cytometry.
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Applications for DPH2 Antibody (OTI4E7) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DPH2

DPH2 is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

diphthamide biosynthesis protein 2, Diphthamide biosynthesis protein 2 homolog-like 2, diptheria toxin resistance protein required for diphthamide biosynthesis-like 2, diptheria toxin resistance protein required for diphthamide biosynthesis-like 2(S. cerevisiae), DPH2 homolog, DPH2 homolog (S. cerevisiae), DPH2L2diphthamide biosynthesis-like protein 2, DPH2-like 2, DPH2-like 2 (S. cerevisiae), HsDph2

Gene Symbol

DPH2

Additional DPH2 Products

Product Documents for DPH2 Antibody (OTI4E7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DPH2 Antibody (OTI4E7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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