DPH2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13933

Novus Biologicals, part of Bio-Techne
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NBP2-13933
NBP2-13933-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DPH2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: DPKAPVVLLSEPACAHALEALATLLRPRYLDLLVSSPAFPQPVGSLSPEPMPLERFGRRFPLAPGRRLEEYGAFYVGGSKASPDPDLDPDLSRL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DPH2 Antibody

Immunocytochemistry/ Immunofluorescence: DPH2 Antibody [NBP2-13933]

Immunocytochemistry/ Immunofluorescence: DPH2 Antibody [NBP2-13933]

Immunocytochemistry/Immunofluorescence: DPH2 Antibody [NBP2-13933] - Immunofluorescent staining of human cell line Hep G2 shows localization to nucleus.
Immunohistochemistry-Paraffin: DPH2 Antibody [NBP2-13933]

Immunohistochemistry-Paraffin: DPH2 Antibody [NBP2-13933]

Immunohistochemistry-Paraffin: DPH2 Antibody [NBP2-13933] - Staining of human testis shows strong nuclear positivity in Leydig cells and subset of cells in seminiferous ducts.

Applications for DPH2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DPH2

DPH2 is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

diphthamide biosynthesis protein 2, Diphthamide biosynthesis protein 2 homolog-like 2, diptheria toxin resistance protein required for diphthamide biosynthesis-like 2, diptheria toxin resistance protein required for diphthamide biosynthesis-like 2(S. cerevisiae), DPH2 homolog, DPH2 homolog (S. cerevisiae), DPH2L2diphthamide biosynthesis-like protein 2, DPH2-like 2, DPH2-like 2 (S. cerevisiae), HsDph2

Gene Symbol

DPH2

Additional DPH2 Products

Product Documents for DPH2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DPH2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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