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DPH5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76567

Novus Biologicals, part of Bio-Techne
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NBP2-76567
NBP2-76567-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VLRATKLGIPYRVIHNASIMNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DPH5 Antibody

Immunocytochemistry/ Immunofluorescence: DPH5 Antibody [NBP2-76567]

Immunocytochemistry/ Immunofluorescence: DPH5 Antibody [NBP2-76567]

Immunocytochemistry/Immunofluorescence: DPH5 Antibody [NBP2-76567] - Staining of human cell line RH-30 shows localization to the Golgi apparatus. Antibody staining is shown in green.

Applications for DPH5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, containing 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DPH5

DPH5 encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CGI-30, Diphthamide biosynthesis methyltransferase, diphthine synthase, DPH5 homolog (S. cerevisiae), EC 2.1.1.98, HSPC143, MGC61450, NPD015, protein x 0011

Gene Symbol

DPH5

Additional DPH5 Products

Product Documents for DPH5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DPH5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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