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DTD1 Antibody (OTI1E1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72335

Novus Biologicals, part of Bio-Techne
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NBP2-72335

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Summary for DTD1 Antibody (OTI1E1) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human DTD1(NP_543010) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

23.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DTD1 Antibody (OTI1E1) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: DTD1 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72335]
Flow Cytometry: DTD1 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72335]

Flow Cytometry: DTD1 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72335]

Flow Cytometry: DTD1 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72335] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DTD1( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DTD1.

Applications for DTD1 Antibody (OTI1E1) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:4000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DTD1

DTD1 is encoded by this gene is similar in sequence to histidyl-tRNA synthetase, which hydrolyzes D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr). The encoded protein is found in the cytoplasm. [provided by RefSeq]

Alternate Names

bA379J5.3, bA555E18.1, C20orf88HARS2, chromosome 20 open reading frame 88, DNA-unwinding element-binding protein, D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae), D-tyrosyl-tRNA(Tyr) deacylase 1, DUEB, EC 3.1, Histidyl-tRNA synthase-related, histidyl-tRNA synthetase 2, MGC119131, MGC41905, pqn-68

Gene Symbol

DTD1

Additional DTD1 Products

Product Documents for DTD1 Antibody (OTI1E1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DTD1 Antibody (OTI1E1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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