Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71331

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2C4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Tyro3/Dtk Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TYRO3 (NP_006284) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

96.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free

Western Blot: Dtk/TYRO3 Antibody (OTI2C4)Azide and BSA Free [NBP2-71331]

Western Blot: Dtk/TYRO3 Antibody (OTI2C4)Azide and BSA Free [NBP2-71331]

Western Blot: Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free [NBP2-71331] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYRO3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3.
Immunocytochemistry/ Immunofluorescence: Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free [NBP2-71331]

Immunocytochemistry/ Immunofluorescence: Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free [NBP2-71331]

Immunocytochemistry/Immunofluorescence: Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free [NBP2-71331] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TYRO3.
Immunohistochemistry: Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free [NBP2-71331]

Immunohistochemistry: Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free [NBP2-71331]

Immunohistochemistry: Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free [NBP2-71331] - Staining of paraffin-embedded Human Ovary tissue using anti-TYRO3 mouse monoclonal antibody.
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Applications for Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunoprecipitation

2ug/500ul

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Tyro3/Dtk

Receptor protein tyrosine kinases (RPTKs) are a class of transmembrane signaling molecules. They have been shown to regulate signaling cascades that control synaptic function in the central nervous system (CNS). Tyro3 is one of three RPTKs belonging to the Axl/UFO subfamily, that also includes Axl and Mer. Tyro3 is the most commonly expressed of these receptors in the CNS. It may be involved in the cell adhesion process, and is commonly found in adult tissues with expression mostly in brain, testis, vascular and lymphatic tissue.

Long Name

Tyrosine Protein Kinase Receptor TYRO3

Alternate Names

Brt, Dtk, Rse, Sky

Gene Symbol

TYRO3

Additional Tyro3/Dtk Products

Product Documents for Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Dtk/TYRO3 Antibody (OTI2C4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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