DTX2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52144
Key Product Details
Species Reactivity
Human, Rat, Bovine
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-EKLSTASGYSDVTD corresponding to internal region according to NP_065943.2, NP_001096066.1.
Epitope
C-EKLSTASGYSDVTD
Reactivity Notes
Expected from sequence similarity: Rat, Bovine
Specificity
This antibody is expected to recognize both reported isoforms (NP_065943.2; NP_001096066.1). Reported variants NP_001096065.1, NP_065943.2, NP_001096064.1 represent identical protein.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for DTX2 Antibody
![Immunohistochemistry-Paraffin: DTX2 Antibody [NBP1-52144]](https://resources.bio-techne.com/images/products/DTX2-Antibody-Immunohistochemistry-Paraffin-NBP1-52144-img0001.jpg "Immunohistochemistry-Paraffin: DTX2 Antibody [NBP1-52144]")
Immunohistochemistry-Paraffin: DTX2 Antibody [NBP1-52144]
Immunohistochemistry-Paraffin: DTX2 Antibody [NBP1-52144] - 5ug/ml staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for DTX2 Antibody
Application
Recommended Usage
Immunohistochemistry
0.3ug/ml
Peptide ELISA
Detection limit 1:32000
Western Blot
detection limit 1:32000
Application Notes
Western blot: Preliminary experiments gave an approx 85kDa band in lysates of cell lines A549, HepG2 and NIH3T3 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.2kDa according to NP_065943.2. The 85kDa band was successfully blocked by incubation with the immunizing peptide
IHC: Paraffin embedded Human Small Intestine.
IHC: Paraffin embedded Human Small Intestine.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: DTX2
Alternate Names
deltex (Drosophila) homolog 2, deltex homolog 2 (Drosophila), Deltex2, hDTX2, MGC71098, protein deltex-2, RING finger protein 58, RNF58KIAA1528deltex2, zinc ion binding protein
Gene Symbol
DTX2
UniProt
Additional DTX2 Products
Product Documents for DTX2 Antibody
Product Specific Notices for DTX2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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