DTX3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46127

Novus Biologicals, part of Bio-Techne
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NBP1-46127
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Bovine (100%), Human (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all DTX3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KNKVTVSKPVWD corresponding to internal region (near N-Terminus) according to NP_848597.1.

Epitope

internal region (near N Terminus)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DTX3 Antibody

Western Blot: DTX3 Antibody [NBP1-46127]

Western Blot: DTX3 Antibody [NBP1-46127]

Western Blot: DTX3 Antibody [NBP1-46127] - (0.3ug/ml) Analysis of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for DTX3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

0.2 - 0.5 ug/ml
Application Notes
WB: Approx. 90 kDa band observed in mouse Fetal brain lysates and in NIH3T3 lysates is reported in scientific literature (PMID: 12670957).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DTX3

DTX3 encodes a probable E3 ubiquitin-protein ligase DTX3 that exists in two isoforms: Isoform 1 id 347 amino acids long at nearly 38 kDA, while isoform 2 is 350 amino acids long at 38 kDA as well. DTX3 is involved in the notch signaling pathway and protein modification and is known to interact with genes UBE2D2, UBE2D3, UBE2L6, UBE2V1, and UBE2W. It is critical in cell to cell communication that affects cell-fate determinations.

Alternate Names

deltex 3 homolog (Drosophila), deltex homolog 3 (Drosophila), Deltex3, FLJ34766, MGC138863, MGC138864, protein deltex-3, RING finger protein 154, RNF154deltex 3 homolog

Entrez Gene IDs

196403 (Human); 80904 (Mouse); 500847 (Rat)

Gene Symbol

DTX3

UniProt

NP_848597.1 (Human)

Additional DTX3 Products

Product Documents for DTX3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DTX3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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