Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all DUSP10 Antibodies »

Product Specifications

Immunogen

maps to a region between residue 432 and 482 of human dual specificity phosphatase 10 using the numbering given in entry NP_009138.1

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DUSP10 Antibody

Immunoprecipitation: DUSP10 Antibody [NBP1-30062]

Immunoprecipitation: DUSP10 Antibody [NBP1-30062]

Immunoprecipitation: DUSP10 Antibody [NBP1-30062] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DUSP10 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. DUSP10 was also immunoprecipitated by rabbit anti-DUSP10 antibody BL5323, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for DUSP10 Antibody

Application
Recommended Usage

Immunoprecipitation

10-15 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DUSP10

Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK, but not MAPK/ERK. Its subcellular localization is unique; it is evenly distributed in both the cytoplasm and the nucleus. This gene is widely expressed in various tissues and organs, and its expression is elevated by stress stimuli. Three transcript variants encoding two different isoforms have been found for this gene.

Alternate Names

dual specificity phosphatase 10, dual specificity phosphatase MKP-5, EC 3.1.3.16, EC 3.1.3.48, MAP kinase phosphatase 5, Mitogen-activated protein kinase phosphatase 5, MKP-5dual specificity protein phosphatase 10, MKP5serine/threonine specific protein phosphatase

Entrez Gene IDs

11221 (Human)

Gene Symbol

DUSP10

UniProt

Q9Y6W6 (Human)

Additional DUSP10 Products

Product Documents for DUSP10 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DUSP10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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