DUSP23 Antibody (OTI3C10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71976

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3C10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all DUSP23 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human DUSP23(NP_060293) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

16.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DUSP23 Antibody (OTI3C10) - Azide and BSA Free

Western Blot: DUSP23 Antibody (OTI3C10)Azide and BSA Free [NBP2-71976]

Western Blot: DUSP23 Antibody (OTI3C10)Azide and BSA Free [NBP2-71976]

Western Blot: DUSP23 Antibody (OTI3C10) - Azide and BSA Free [NBP2-71976] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DUSP23 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DUSP23.
Immunocytochemistry/ Immunofluorescence: DUSP23 Antibody (OTI3C10) - Azide and BSA Free [NBP2-71976]

Immunocytochemistry/ Immunofluorescence: DUSP23 Antibody (OTI3C10) - Azide and BSA Free [NBP2-71976]

Immunocytochemistry/Immunofluorescence: DUSP23 Antibody (OTI3C10) - Azide and BSA Free [NBP2-71976] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DUSP23.
Immunohistochemistry: DUSP23 Antibody (OTI3C10) - Azide and BSA Free [NBP2-71976]

Immunohistochemistry: DUSP23 Antibody (OTI3C10) - Azide and BSA Free [NBP2-71976]

Immunohistochemistry: DUSP23 Antibody (OTI3C10) - Azide and BSA Free [NBP2-71976] - Staining of paraffin-embedded Human prostate tissue using anti-DUSP23 mouse monoclonal antibody.
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Applications for DUSP23 Antibody (OTI3C10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DUSP23

DUSP23 is a dual substrate specificity phosphatase that mediates dephosphorylation of proteins phosphorylated on Tyr and Ser/Thr residues. It is widely expressed and highly conserved. Most of dual-specificity protein phosphatases (DSPs) play an important role in the regulation of mitogenic signal transduction and controlling the cell cycle in response to extracellular stimuli. Reverse transcription-PCR (RT-PCR) revealed that the DUSP23 was expressed in most fetal tissues and two adult tissues: testis and colon.

Alternate Names

dual specificity phosphatase 23, dual specificity protein phosphatase 23, DUSP25, EC 3.1.3.16, EC 3.1.3.48, FLJ20442, LDP3, LDP-3, Low molecular mass dual specificity phosphatase 3, low-molecular-mass dual-specificity phosphatase 3, MOSP, RP11-190A12.1, VH1-like member Z, VH1-like phosphatase Z, VHZ

Gene Symbol

DUSP23

Additional DUSP23 Products

Product Documents for DUSP23 Antibody (OTI3C10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DUSP23 Antibody (OTI3C10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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