DUX4 Antibody (P2B1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12886
![Western Blot: DUX4 Antibody (P2B1) [NBP2-12886]](https://resources.bio-techne.com/images/products/DUX4-Antibody-P2B1-Western-Blot-NBP2-12886-img0005.jpg "Western Blot: DUX4 Antibody (P2B1) [NBP2-12886]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # P2B1
Concentration
1 mg/ml
Product Specifications
Immunogen
C-terminal 76 amino acids of DUX4 with glutathione-s-transferase (gst) tag
Localization
Nucleus
Specificity
Detects approx 45kDa. No cross-reactivity with DUX4c.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for DUX4 Antibody (P2B1)
Western Blot: DUX4 Antibody (P2B1) [NBP2-12886]
Western Blot: DUX4 Antibody (P2B1) [NBP2-12886] - analysis of Mouse C2C12 cell lysate showing detection of DUX4 protein using Mouse Anti-DUX4 Monoclonal Antibody, Clone P2B1 . Primary Antibody: Mouse Anti-DUX4 Monoclonal Antibody at 1:1000. Cells transfected with pCS2+DUX4 which, contains an additional upstream start site.![Immunocytochemistry/ Immunofluorescence: DUX4 Antibody (P2B1) [NBP2-12886]](https://resources.bio-techne.com/images/products/DUX4-Antibody-P2B1-Immunocytochemistry-Immunofluorescence-NBP2-12886-img0004.jpg "Immunocytochemistry/ Immunofluorescence: DUX4 Antibody (P2B1) [NBP2-12886]")
Immunocytochemistry/ Immunofluorescence: DUX4 Antibody (P2B1) [NBP2-12886]
Immunocytochemistry/Immunofluorescence: DUX4 Antibody (P2B1) [NBP2-12886] - Tissue: C2C12 myoblast cells. Species: Mouse. Primary Antibody: Mouse Anti-DUX4 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain.Applications for DUX4 Antibody (P2B1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:1000
Application Notes
1 ug/ml of DUX4 Antibody was sufficient for detection of DUX4 in 20 ug of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG: HRP as the secondary.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DUX4
Long Name
Double Homeobox 4
Alternate Names
DUX10, DUX4L1
Gene Symbol
DUX4
Additional DUX4 Products
Product Documents for DUX4 Antibody (P2B1)
Product Specific Notices for DUX4 Antibody (P2B1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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