DYKDDDDK Epitope Tag Antibody (C.15200054)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59146

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Epitope Tag

Applications

Chromatin Immunoprecipitation (ChIP), Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # C.15200054

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DYKDDDDK Epitope Tag Antibodies »

Product Summary for DYKDDDDK Epitope Tag Antibody (C.15200054)

Immunogen

The exact immunogen of DYKDDDDK Epitope Tag Antibody (C.15200054) is propietary information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

1.01 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DYKDDDDK Epitope Tag Antibody (C.15200054)

Western Blot: DYKDDDDK Epitope Tag Antibody (C.15200054) [NBP2-59146]

Western Blot: DYKDDDDK Epitope Tag Antibody (C.15200054) [NBP2-59146]

Western Blot: DYKDDDDK Epitope Tag Antibody (C.15200054) [NBP2-59146] - Analysis of 40 ug whole cell extracts from HeLa cells transfected with either Ty1-tagged MBD1 (lanes 1 and 3) or Ty1-tagged MBD2 (lane 2 and 4). The WB was performed with the DYKDDDDK Epitope Tag Antibody (C.15200054) [NBP2-59146] diluted 1:1000 in TBS-Tween containing 5% skimmed milk (lane 1 and 2) or with an MBD1 antibody (lane 3) or an MBD2 antibody (lane 4). The position of the Ty1-tagged MBD1 and Ty1-tagged MBD2 are shown on the right, the marker (kDa) is shown on the left.
Chromatin Immunoprecipitation: DYKDDDDK Epitope Tag Antibody (C.15200054) [NBP2-59146] - Results obtained with the [NBP2-59146]. ChIP assays were performed using Plasmodium falciparium cells stably expressing Ty1-H2A (blue bars) and wild type Plasmodium falciparium cells (brown bars). Primer pairs were used for active genes, inactive genes, and variable genes. Sheared chromatin from 1x10^6 cells and 2 ug of antibody against Ty1 were used per ChIP experiment.

Applications for DYKDDDDK Epitope Tag Antibody (C.15200054)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

2 ug/IP

Western Blot

1:1000 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DYKDDDDK Epitope Tag

Fusion proteins, in which a known peptide such as a His-Tag, GST-Tag, or DYKDDDDK (FLAG(R)) is cloned with the target of interest, were developed to simplify protein isolation. Since specific antibodies may not be available against the protein of interest, epitope tag antibodies are commonly used to detect protein expression.

The epitope tag, DYKDDDDK, is comprised of an eight amino acid sequence derived from the gene-10 product from bacteriophage T7 and contains an enterokinase-cleavage site (1). Its hydrophilic nature and short peptide sequence reduce possible interference with protein expression, post-translational modifications, protein interactions, and protein function (2). The DYKDDDDK (FLAG(R)) epitope tag can either be fused to the N-terminus, C-terminus, or placed at an internal position of the target protein, facilitating characterization of the protein of interest using immunodetection methods. Compared to the standard DYKDDDDK epitope tag antibody, the rat monoclonal DYKDDDDK Epitope Tag antibody (clone L5) is 2-8 times more sensitive (3).

References

1. Brizzard BL, Chubet RG, Vizard DL. (1994) Immunoaffinity purification of (FLAG(R)) epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution. Biotechniques. 16(4):730-5. PMID: 8024796

2. Einhauer A, Jungbauer A. (2001) The (FLAG(R)) peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 49(1-3):455-65. PMID: 11694294

3. Park SH, Cheong C, Idoyaga J, Kim JY, Choi JH, Do Y, Lee H, Jo JH, Oh YS, Im W, Steinman RM, Park CG. (2008) Generation and application of new rat monoclonal antibodies against synthetic DYKDDDDK Epitope Tag (FLAG(R)) and OLLAS tags for improved immunodetection. J Immunol Methods. 331(1-2):27-38. PMID: 18054954

FLAG(R) is a trademark of Sigma-Aldrich.

Alternate Names

FLAG Tag

Additional DYKDDDDK Epitope Tag Products

Product Documents for DYKDDDDK Epitope Tag Antibody (C.15200054)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DYKDDDDK Epitope Tag Antibody (C.15200054)

FLAG(R) and ANTI-FLAG(R) are registered trademarks of Sigma-Aldrich Co. LLC.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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