DYKDDDDK Epitope Tag Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37833
Key Product Details
Species Reactivity
Validated:
Epitope Tag
Applications
Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1 mg/ml
Product Summary for DYKDDDDK Epitope Tag Antibody
Immunogen
DYKDDDDK Epitope Tag Antibody was made to a Synthetic Peptide: D Y K D D D D K C
Specificity
DYKDDDDK Epitope Tag Antibody detects the DYKDDDDK epitope tag sequence.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
1.01 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DYKDDDDK Epitope Tag Antibody
![Western Blot: DYKDDDDK Epitope Tag Antibody [NBP2-37833]](https://resources.bio-techne.com/images/products/DYKDDDDK-Epitope-Tag-Antibody-Western-Blot-NBP2-37833-img0002.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody [NBP2-37833]")
Western Blot: DYKDDDDK Epitope Tag Antibody [NBP2-37833]
Western Blot: DYKDDDDK Epitope Tag Antibody [NBP2-37833] - Analysis of recombinant protein containing the epitope tag sequence DYKDDDDK using DYKDDDDK Epitope Tag Antibody [NBP2-37833].Applications for DYKDDDDK Epitope Tag Antibody
Application
Recommended Usage
Immunofluorescence
1 ug/ml
Western Blot
1:500
Application Notes
DYKDDDDK Epitope Tag Antibody has been successfully used in Western blot and immunofluorescence procedures to detect the presence of fusion proteins containing the epitope tag sequence DYKDDDDK.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: DYKDDDDK Epitope Tag
The epitope tag, DYKDDDDK, is comprised of an eight amino acid sequence derived from the gene-10 product from bacteriophage T7 and contains an enterokinase-cleavage site (1). Its hydrophilic nature and short peptide sequence reduce possible interference with protein expression, post-translational modifications, protein interactions, and protein function (2). The DYKDDDDK (FLAG(R)) epitope tag can either be fused to the N-terminus, C-terminus, or placed at an internal position of the target protein, facilitating characterization of the protein of interest using immunodetection methods. Compared to the standard DYKDDDDK epitope tag antibody, the rat monoclonal DYKDDDDK Epitope Tag antibody (clone L5) is 2-8 times more sensitive (3).
References
1. Brizzard BL, Chubet RG, Vizard DL. (1994) Immunoaffinity purification of (FLAG(R)) epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution. Biotechniques. 16(4):730-5. PMID: 8024796
2. Einhauer A, Jungbauer A. (2001) The (FLAG(R)) peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 49(1-3):455-65. PMID: 11694294
3. Park SH, Cheong C, Idoyaga J, Kim JY, Choi JH, Do Y, Lee H, Jo JH, Oh YS, Im W, Steinman RM, Park CG. (2008) Generation and application of new rat monoclonal antibodies against synthetic DYKDDDDK Epitope Tag (FLAG(R)) and OLLAS tags for improved immunodetection. J Immunol Methods. 331(1-2):27-38. PMID: 18054954
FLAG(R) is a trademark of Sigma-Aldrich.
Alternate Names
FLAG Tag
Additional DYKDDDDK Epitope Tag Products
Product Documents for DYKDDDDK Epitope Tag Antibody
Product Specific Notices for DYKDDDDK Epitope Tag Antibody
FLAG(R) and ANTI-FLAG(R) are registered trademarks of Sigma-Aldrich Co. LLC.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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