Dynactin Subunit 2/DCTN2/DCTN-50 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85277

Novus Biologicals, part of Bio-Techne
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NBP1-85277
NBP1-85277-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Dynactin Subunit 2/DCTN2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody

Knockdown Validated: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Knockdown Validated: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Knockdown Validated: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277] - Quantification of Dynactin-2 knockdown in HeLa cells by Western Blot. Western blots were cropped as indicated (black boxes). For all samples, SDS-PAGE and Western blotting were performed under the same experimental conditions. n:20 for each condition. * = p < 0.05; ** = p < 0.01. Scale bar 10 um. Error bars represent SEM. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep08389) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Immunocytochemistry/ Immunofluorescence: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Immunocytochemistry/Immunofluorescence: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277] - Dynactin-2 is part of the linker arm for the molecular motor Dynein. ILK and Dynactin-2 colocalize at the basal cortex but not at the spindle. of a metaphase cell. Scale bar 10 um. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep08389) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277]

Immunohistochemistry-Paraffin: Dynactin Subunit 2/DCTN2/DCTN-50 Antibody [NBP1-85277] - Staining of human cerebral cortex, colon, kidney and testis using Anti-DCTN2 antibody NBP1-85277 (A) shows similar protein distribution across tissues to independent antibody NBP1-85278 (B).
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Applications for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF and WB reactivity reported in (PMID: 25669897).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dynactin Subunit 2/DCTN2

Dynactin subunit 2 encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place.

Alternate Names

DCTN2, Dynamitin, RBP50

Gene Symbol

DCTN2

Additional Dynactin Subunit 2/DCTN2 Products

Product Documents for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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