Dynamin 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47477

Novus Biologicals, part of Bio-Techne
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NBP2-47477
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Dynamin 2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PPQIPSRPVRIPPGIPPGVPSRR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dynamin 2 Antibody

Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477]

Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477]

Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Analysis in human colon and liver tissues. Corresponding Dynamin 2 RNA-seq data are presented for the same tissues.
Western Blot: Dynamin 2 Antibody [NBP2-47477]

Western Blot: Dynamin 2 Antibody [NBP2-47477]

Western Blot: Dynamin 2 Antibody [NBP2-47477] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.
Immunocytochemistry/ Immunofluorescence: Dynamin 2 Antibody [NBP2-47477]

Immunocytochemistry/ Immunofluorescence: Dynamin 2 Antibody [NBP2-47477]

Immunocytochemistry/Immunofluorescence: Dynamin 2 Antibody [NBP2-47477] - Staining of human cell line HeLa shows localization to the Golgi apparatus. Antibody staining is shown in green.
View 4 more images

Applications for Dynamin 2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Knockout validation (PMID: 31390291).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dynamin 2

The dynamins are a family of 100 kDa GTPases transcribed from at least three separate genes. At least four mRNA splice variants for each dynamin have been described. Dynamins contain several conserved regions including the conserved, amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a coiled-coil region located in front of a proline-rich domain (PRD). The PRD is thought to mediate interactions between dynamin and numerous other cellular proteins. Dynamin 1 is expressed exclusively in neurons, Dynamin 2 is ubiquitously expressed, and Dynamin 3 is thought to be restricted to expression in the brain, testis, heart, and lung. The dynamins participate in the cellular process of clathrin-mediated and fluid-phase endocytosis.

Long Name

Dynamin-2

Alternate Names

DNM2, DYN2

Gene Symbol

DNM2

Additional Dynamin 2 Products

Product Documents for Dynamin 2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Dynamin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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