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Dynamin Antibody (A1B1-R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32282

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32282

Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # A1B1-R

Concentration

1 mg/ml

Product Summary for Dynamin Antibody (A1B1-R)

Immunogen

Recombinant protein within human Dynamin aa 500-800. (Uniprot: Q05193)

Localization

Cytoskeleton, cytoplasm.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dynamin Antibody (A1B1-R)

Dynamin Antibody (A1B1-R)

Western Blot: Dynamin Antibody (A1B1-R) [NBP3-32282]

Western Blot: Dynamin Antibody (A1B1-R) [NBP3-32282] - Western blot analysis of Dynamin on different lysates with Mouse anti-Dynamin antibody (NBP3-32282) at 1/1,000 dilution.

Lane 1: Rat brain tissue lysate
Lane 2: Mouse brain tissue lysate

Lysates/proteins at 40 ug/Lane.

Predicted band size: 97 kDa
Observed band size: 100 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32282) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for Dynamin Antibody (A1B1-R)

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dynamin

The dynamin family of GTPases is essential for receptor-mediated endocytosis and synaptic vesicle recycling, and it has recently been shown to play a role in vesicle formation from the trans-Golgi network (1). Dynamin-1 is a GTP-, microtubule-, and phospholipid-binding protein that is expressed primarily in brain and almost exclusively expressed in the central nervous system (2). Dynamin-1 is believed to assemble around the necks of clathrin-coated pits and assist in pinching vesicles from the plasma membrane. This role would make dynamin unique among GTPases in its ability to act as a mechanochemical enzyme (1). Dynamin-1 function is apparently essential for scission of newly formed vesicles from the plasma membrane; it has been proposed that dynamin is a regulator of downstream effectors of scission, rather than being directly responsible for it (3).

Alternate Names

DNM1

Gene Symbol

DNM1

Additional Dynamin Products

Product Documents for Dynamin Antibody (A1B1-R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Dynamin Antibody (A1B1-R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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